LC Irene Finney

cattleyas: a perspective from Japan

Rlc. Shinfong Beauty‘Shinfong Beauty（新豊美人）

DEFLASKING seedlings

Individual - estufa

AB1

A6 - enraizamento

Considerações MEIO

http://home.uevora.pt/~zavattieri/meios_de_cultura.htm

Flasking Medium for Phalaenopsis Seed Sowing & Seedlings

These are a couple of formula's that a number of us have been using for Phalaenopsis.  The mother media was designed for sowing seeds with the green pod method, but it will also work with dry harvested seed.  Phals generally prefer a medium with weaker nutrients than recommended amount of most commercial flasking media.   So the amounts listed below are tailored specifically for Phalaenopsis.  Phals are also sensitive to sugar when temperatures are higher, if you find your temps run higher in your lab and you are getting die-off, try reducing the amount of sugar.

Mother Media Formula

13.5g/L    P668 (Phytotech Orchid Maintenance Media with Charcoal)
5g/L         Sugar
3-5g/L      Gelling Agent
1L            Distilled Water
 

Replate Media Formula

20g/L       P668 (Phytotech Orchid Maintenance Media with Charcoal)
5g/L         Sugar
25g/L       Banana
50g/L       Potato (cooked and mashed)
3-5g/L      Gelling Agent
950ml/L   Distilled Water

Directions:  Puree the Potato and Banana in a blender with some of the distilled water. Mix all the ingredients together and check the pH.  pH should be around 6.  The P668 media is usually self adjusting, but adding the Banana and Potato can throw off the pH.  Adjust it if needed.   Once that is done bring the mix to a rolling boil.  Then flasks can be filled and autoclaved.

Source: http://orchidvault.com/node/72 on September 1,2011 at 16:02 Brazilian Local time